An extensive international study published in Nature reveals the complexities of the human "4D nucleome," detailing how its three-dimensional genome organization affects gene regulation and DNA replication across various cell states. Conducted by the NIH-funded 4D Nucleome Project, it compared multiple genome architecture assays, uncovering over 140,000 chromatin loops in stem cells and fibroblasts. The findings offer crucial insights for laboratories on assay selection and lay a foundation for predicting genome folding and interpreting genetic variants' roles in gene regulation.
1. Study focuses on the human 4D nucleome. 2. Examines embryonic stem cells and fibroblasts. 3. Over 140,000 chromatin loops identified. 4. Systematic comparison of genome architecture assays. 5. Different assays capture various aspects of genome organization. 6. Integrated data outline spatial genome states. 7. Correlation with gene expression and histone modifications. 8. Models predict genome folding from DNA sequences.
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