Antibody Oxidation’s Hidden Handedness Comes Into View - Summary - MDSpire
Feature

Antibody Oxidation’s Hidden Handedness Comes Into View

Integrated NMR and LC-MS analysis distinguishes elusive stereochemical forms of methionine oxidation in IgG1 Fc regions, offering a sharper tool for biologic drug quality control

  • April 8, 2026

  • 2 min

Share

Researchers have developed an innovative strategy combining methyl-based NMR spectroscopy, selective enzymatic reduction, and LC-MS peptide mapping to analyze the stereochemical variations of methionine oxidation in IgG1 antibodies. Focusing on the conserved Fc methionines Met252 and Met428, the study reveals how oxidation impacts antibody stability and receptor interactions. This method enables the differentiation of R and S stereoisomers, providing insights that could enhance stability testing and manufacturing controls for therapeutic antibodies.

Original Source(s)

Antibody Oxidation’s Hidden Handedness Comes Into View

  • April 8, 2026

Related Content

Small Phase II Study Examines Triplet Regimen for Patients With HER2-Positive Breast Cancer and Leptomeningeal Metastasis

Results from a small phase II study suggest that a triplet regimen may improve symptoms and extend survival in some patients with breast cancer and leptomeningeal metastasis, who often face limited tr...

Analysis Shows Nearly 7% of Global Clinical Trials Are Impacted by Middle East Disruption

Oncology trials account for a substantial proportion of impacted activity, with non–small cell lung cancer representing the most affected indication, followed by breast cancer.

Perioperative Enfortumab Vedotin Plus Pembrolizumab in Cisplatin-Ineligible Bladder Cancer

As reported in The New England Journal of Medicine by Vulsteke et al, the phase III KEYNOTE-905/EV-303 trial has shown improved event-free and overall survival with the addition of perioperative enfor...